Atf4 kda
WebATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of … WebAnti-ATF4 Antibody Anti-ATF4 is an antibody targeting the ATF4 protein, validated for use in WB & ChIP. - Find MSDS or SDS, a COA, data sheets and more information. United States / English; Quick Purchase; My Favorites ... The calculated molecular weight is 39 kDa; however, this protein may be observed at ~48 kDa in human tissue lysates and ~45 ...
Atf4 kda
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WebBackground: ATF4. ATF4 is an approximately 38 kDa member of the bzip family of transcription factors. It plays an important role autophagy, the unfolded protein response, … WebDec 28, 2024 · The Golgi apparatus is involved in the intracellular transport and maturation of proteins and lipids (Rohn et al., 2000; Viotti, 2016).More than a third of all human genes are known to encode proteins that travel through the Golgi (Yuen et al., 1997).The Golgi has a distinctive structure with several layers of flat, semicircular vesicles known as cisternae.
WebMar 28, 2024 · Methods. Hepatocyte-specific ATF4 deficient MUP-uPA mice (MUP-uPA/Atf4 Δhep) and control MUP-uPA/Atf4 F/F mice were fed high fat diet (HFD) to induce NASH-induced HCC, and Atf4 F/F and Atf4 Δhep mice were injected with diethylnitrosamine (DEN) to model carcinogen-induced HCC. Histological, biochemical, … WebWestern Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4 protein using Mouse Anti-ATF4 Monoclonal Antibody, Clone S360A-24 (SMC-447). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody (SMC-447) at 1:1000 …
WebJan 25, 2024 · Mesenchymal stem cells (MSCs) could be a promising solution in the treatment of various diseases including chronic kidney disease (CKD). However, endoplasmic reticulum (ER) stress induced by ischemia in the area of application limits the integration and survival of MSCs in patients. In our study, we generated ER stress … WebTM3 cells were treated without or with different concentrations of midazolam (150 and 300 μM) for 3, 6, 12 and 24 hours. PERK (140 kDa), phosphor-EIF2α (38 kDa), EIF2α (36 kDa), ATF4 (49 kDa), ATF3 (21 kDa) and CHOP (30 kDa) were detected by Western blot analysis (A). Immunoblot represents the observations from one single experiment repeated ...
WebPurpose: To determine concentrations of endoplasmic reticulum (ER) stress-related factors activating transcription factor 4 (ATF4) and glucose-regulated 78 kDa protein (GRP78) in …
WebChIP. Chromatin immunoprecipitations were performed with cross-linked chromatin from MEF wild-type cells treated with tunicamycin (2ug/ml) overnight, and CHOP (L63F7) Mouse mAb #2895 or Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using … git merge and squashWebMay 31, 2010 · This suggests that the HIF and BCL2/adenovirus E1B 19-kDa protein-interacting protein 3 pathway may be less important under chronic and severe hypoxia … git merge another branch into currentgit merge and rebase differenceWeb1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF4 (60035-1-Ig, Clone:3E4C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at … git merge a single commit from another branchWebDec 10, 2016 · C/EBP-homologous protein, CHOP or GADD153 (growth arrest and DNA damage 153), is another protein induced under conditions of severe ER stress by XBP1 and ATF4 (another UPR component). The 29 kDa CHOP is a member of the C/EBP family of transcription factors. furniture in bloomington mnWebMar 22, 2024 · Moreover, the secretion of CCL2 in cancer milieu is regulated by activating transcription factor 4 (ATF4), ... (IFN) γ-induced protein; IP-10) is a 10 kDa protein functionally classified as a Th1 chemokine. It binds to the CXCR3 receptor and regulates the immune response by activating inflammatory cells such as T lymphocytes, eosinophils, … furniture in burlington vtWebMay 12, 2024 · ABSTRACT. Purpose: To determine concentrations of endoplasmic reticulum (ER) stress-related factors activating transcription factor 4 (ATF4) and glucose-regulated 78 kDa protein (GRP78) in vitreous and aqueous humor (AqH) of patients with proliferative diabetic retinopathy (PDR) and the correlation of ATF4, GRP78 and inflammatory … furniture in brookhaven